Micro-domains as site for storage lipid biosynthesis in seeds and algae
- Brand: Bruckhoff, Dr. Daniel
- Availability: In stock
- SKU: 9783863761714
Every cell is capable of synthesizing and storing triacylglycerols (TAGs) as carbon and/or energy source. Although the biosynthetic pathways that lead to the formation of TAG are well established, knowledge of the factors that determine the amount of TAG to be synthesized in a cell is still scarce. Thus, the...
Every cell is capable of synthesizing and storing triacylglycerols (TAGs) as carbon and/or energy source. Although the biosynthetic pathways that lead to the formation of TAG are well established, knowledge of the factors that determine the amount of TAG to be synthesized in a cell is still scarce.
Thus, the overall aim of this thesis is to analyze the role of sub- or micro-domains of the ER and lipid droplets (LD) in TAG formation and to use the knowledge gained to achieve an increase in the TAG content in plants and algae. The flowering plant Arabidopsis thaliana and the monocellular microalgae Phaeodactylum tricornutum will serve as model organisms for this study and two different homologs of Arabidopsis acyl-CoA:diacylglycerol acyltransferases DGAT1+ 3), the key enzymes of the TAG synthesis, are targets to influence the TAG accumulation in these organisms.
The central hypothesis that will be tested is the assumption that the flux into TAG biosynthesis can be increased by reallocating its central steps in artificial micro-domains either at the ER or directly to LDs. Therefore, different combinations of enzymes were overexpressed as fusion proteins with oleosin that targets these proteins to the LD membrane. The functionality of the fusion proteins was confirmed via expression in appropriate yeast mutants. Finally TAG accumulation was tested in seeds and P. tricornutum.
- Title: Micro-domains as site for storage lipid biosynthesis in seeds and algae
- Author: Dr. Daniel Bruckhoff
- Edition: 1st edition
- University: Georg-August University of Göttingen
- Published: 1st edition 11.12.2015
- Subject: Biology
- Product Type: Book (Hardcover)
- Product type: Dissertation
- Language: German
- Binding: Softcover (paperback)
- Dimensions: 21.0 x 14.8 cm (DIN A5)
- Extent: XII / 168 pages
- Condition: New (shrink-wrapped in foil)
- Keywords: ACBP6, Arabidopsis thaliana, DGAT1, lipid, microalgae, OLEO3, storage lipids, TAG
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